Agilent Technologies, Inc.
NovoCyte® Advanteon™ Benchtop Flow Cytometer
Advancing the Boundaries of Flow Cytometry
The Agilent NovoCyte Advanteon flow cytometer builds upon the highly successful Agilent NovoCyte and NovoCyte Quanteon to provide an advanced set of capabilities for the most demanding scientists, yet is remarkably simple to operate. The NovoCyte Advanteon can accommodate today’s high-end and increasingly sophisticated multicolor flow cytometry assays, and provides the flexibility of 1, 2, or 3 laser options, with up to 21 fluorescence channels and 23 independent detectors.
NovoCyte Advanteon Flow Cytometer Features
The Ultimate Photodetector
Silicon photomultipliers (SiPM) are solid-state, photon-level sensitive semiconductor devices. The innovative optical design with the NovoCyte Advanteon incorporates 21 independent SiPM, which collect light on each and every channel, with sensitivities that far exceed the traditional PMT’s.
SiPM's strengths:
- Superior photon detection sensitivity
- High gain and high quantum efficiency
- Instantaneous warm up and fast response
- Robust and long lifespan
- High durability
Consistent results, fast or slow
The fluidic feedback control mechanisms found on the NovoCyte Advanteon consistently maintain exceptionally steady flow rates. The superior stability across a wide range of sample flow rates provide consistent results under variable operating conditions. As shown below, the CV’s at different flow rates are dramatically improved over other systems on the market.
Advantage of Performance and Flexibility
With up to 21 fluorescence channels, the NovoCyte Advanteon offers tremendous flexibility in panel design, thereby allowing more data to be collected per run. Assessment of T cell state and function is just one critical example for researching cell-based immunotherapies using multi-color staining panels. Here, the NovoCyte Advanteon was used to show T cell differentiation, monitor T cell exhaustion, and measure cytokine proliferation from PBMC’s following activation, all in one experiment.
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