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CytoTronics, Inc.

Pixel Systems – Visualize live cell function at scale

Pixel™ enables high-resolution, multiplexed, real-time assessment of live-cell characteristics, providing deeper understanding of how chemical or genetic perturbation affect cell function.

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The Pixel System

Pixel is a system of plate and reader options designed to suit your application and utilizes secure cloud-based data processing for real-time monitoring of your experiments.  Users can implement a Pixel Primo for low-throughput applications, a Pixel Octo for high-throughput, or run both in parallel to screen tens of thousands of wells simultaneously.

  • Pixel Primo
    A single plate reader for your incubator
  • Pixel Octo
    A dedicated benchtop incubator with eight plate reader
  • Pixel Plates
    Electronic 96 & 384 well microplates
  • Pixel Pro
    Private cloud-based control and interactive software

Applications

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  • Cancer Cell Biology
    Explore cancer cell behavior through changes in cell viability, migration, in vitro metastasis assays and immuno-oncology applications.

  • Cardiac Function
    Enhance cardiotoxicity studies by enabling simultaneous assessment of both structural and functional toxicity. Model cardiac diseases by measuring interactions between diverse cell populations.

  • Endothelial and Epithelial Cells
    Achieve unparalleled accuracy in real-time monitoring of barrier formation, wound healing, and migration in cell sheets. This capability finds applications in studying intestinal barriers, endothelial cell function and blood-brain barrier studies.

  • Neuron Function
    Investigate neuron function at a single-cell resolution using customizable stimulation protocols. Simultaneously measure extracellular action potential, cell viability, morphology, and metabolism.

  • Stem Cell Biology
    Utilize label-free measurements for continuous monitoring of morphological and functional features of cells over extended periods, spanning days and weeks. Evaluate the pluripotency of cells and track their differentiation into various lineages.

  • Mechanism of Action/Toxicology
    Leverage multi-parametric measurements to generate phenotypic signatures linked to cellular pathways. Identify mechanisms of action (MoAs), fine-tune Structure-Activity Relationship (SAR) studies, and promptly eliminate off-target effects.

A breakthrough in semiconductor-to-cell-biology interface

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CytoTronics takes traditional electrode-based measurements to unprecedented levels of accuracy and scale using microelectrode arrays and integrated circuits to assess live cell functions in real time at single-cell resolution.

Powered by microchips
At the core of the Pixel system is a proprietary microplate embedded with custom-designed semiconductor microchips at the base, providing thousands of measurement and stimulation electrodes in each well.  The electrodes can monitor cell viability, morphology, electrophysiology, metabolism, and more while generating electrical images of single-cell spatial resolution. The system is designed for ease of use by enabling plating of any cell type with a wide range of biological coatings.

Multi-modal insights

The NovoCyte Penteon and NovoCyte Quanteon's fluidic system is designed to deliver high performance. When compared to other flow cytometers, the fluidic consistency and stability of the NovoCyte Penteon and NovoCyte Quanteon is unmatched. Other instruments utilizing peristaltic pumps are often subject to fluidic pulsation, causing inconsistency and inaccuracy in absolute cell counts.

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Monitor cell behavior in real-time

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The Pixel Pro cloud-based application software is designed for interactive analysis and reporting. With the Pixel System, scientists can quickly assess data obtained from every scan to reveal a more complete picture of cell behavior.

Addressing a wide range of cell biology needs

Pixel™ enables functional and morphological mapping of live cells, combining readouts from multiple assays into one.

(Please watch video below)

Videos

News & Events

News

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CytoTronics, Inc.

CytoTronics’ Pixel System Receives 2024 R&D 100 Award

August 27, 2024 – CytoTronics, Inc. has been honored with a 2024 R&D 100 Award. Pixel’s innovative semiconductor-to-live-cell interface empowers cell biologists to re-imagine their experiments and unlock new applications that haven’t been possible before. Early adopters around the world are harnessing Pixel in therapeutic areas including vascular, inflammatory, cardiovascular, and neurological disease, rare disease, and oncology.

Read the Full Press Release

Resources

Please visit CytoTronics' Resources Website for Posters, Pubilations, Application Notes, Technical Notes etc.

Request a demo or a quote

Measure mitochondrial respiration and glycolysis as well as ATP production rate of live cells.

Measure mitochondrial respiration, glycolysis, and ATP production in live cells in a 96-well format

Overview of Consumables for XF Analyzers

Simple, Automated, Charge-based DNA and RNA Extraction

LUNA-FX7™ Automated Fluorescence Cell Counter with unmatched cell counting accuracy

LUNA-III™ Automated Brightfield Cell Counter, designed to enhance your productivity and precision.

High multiplexing, high resolution in situ platform to combine single-cell and spatial genomics.

The best super-resolution microscopy experience, on your desktop.

NovoCyte Opteon Spectral Flow Cytometer Systems 3-5 Lasers and 73 detectors

The NovoCyte Penteon™ is a 5 laser benchtop flow cytometer with up to 30 colors

Pixel – Visualize live cell function at scale

Discover the World's First Two-Laser Image-guided Cell Sorter

Benchtop Microfluidic Cell Sorting with Two Lasers and up to Nine Colors

An incubator designed specifically for the cultivation of primary human cells

Seahorse XFe24 Analyzers measure OCR and ECAR of live cells in a 24-well plate format.

Automated high content image acquisition and analysis for drug discovery and cell biology

Multicolor fluorescence imaging to data analysis in one device

IsoLight – Automated Cellular Proteomics Hub

IsoLight and IsoSpark – Single-Cell Functional Proteomics Hub

The LUNA-FL™ can count in both brightfield and fluorescence mode

The LUNA-II™ automated cell counter gives you the ultimate brightfield cell counting experience.

The MARS® System is the “one-stop” modular solution for complete cell isolation from complex samples

The NovoCyte Quanteon™ is a 4 laser benchtop flow cytometer with up to 25 colors

The QUANTOM Tx Microbial Cell Counter automatically identifies and counts individual bacterial cells

Single Cells or Nuclei from Solid Tissues in Minutes

Tapestri® Platform: Untangle the complexity of cancer with true multi-omics.

Sterile, easy-to-use solutions for high cell integrity of single cell dispensing

Tissue Clearing System for electrophoretic tissue clearing

Optimized Microplate Solutions for Research & Drug Discovery

DeepLabel™ Antibody Staining Kit for Cleared Tissues

IsoPlexis Solutions and Kits

Autofocusing cell counting and declustering algorithm that counts individual yeast cells

Automated Fluorescence Cell Counter for Stem Cells & SVF

The NovoCyte Advanteon provides the flexibility of 1, 2, or 3 laser options.

Accurate & efficient single-cell detection of SNVs, indels, CNVs and LOH across targeted gene panels

Measure functional metabolism in 3 dimensions from in vitro spheroids

NovoCyte Consumables and Accessories for your Flow Cytometry needs.

Kits and reagents for real-time fluorescence plate reader-based live cell metabolism assays

Sterile, easy-to-use solutions for high cell integrity of single cell dispensing

The best value benchtop flow cytometer with high flexibility, enhanced sensitivity and resolution.

Optimized solution for assessing mitochondrial function.

Provides real-time measurements of glycolysis

Measures human (Hu) T cell activation response within several minutes of stimulation

Formulated media based on common culture media DMEM or RPMI, and optimized for use in XF assays.

Analytical method for measuring basal state mitochondrial fuel oxidation in live cells

Identification of drug-induced mitochondrial toxicity

Provides validated reagents to measure long chain fatty acid oxidation (FAO)

For in vitro studies of long chain fatty acid oxidation by intact cells.

Permeabilizes intact cells in culture, selectively targeting the cellular plasma membrane.

Seahorse XF Real-Time ATP Rate Assay

A suite of optimized solutions for rapidly measuring cellular substrate oxidation

Robust & accurate measurements of both glycolytic and mitochondrial activities in T cell populations

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