Logos Biosystems Inc.
LUNA-FL™ Dual Fluorescence Cell Counter
Equipped with dual fluorescence and brightfield optics, the LUNA-FL™ is a powerhouse. The LUNA-FL™ can assess cell counts, viability, and GFP transfection efficiency without being limited by cell type or size.
Technology
With four counting modes, the LUNA-FL™ is our powerhouse. Equipped with brightfield and dual fluorescence optics that allows the sensitive detection of most cell types excluding bacteria. The LUNA-FL™ can distinguish primary cells such as PBMCs, splenocytes, neutrophils, and stem cells from undesirable debris for accurate cell count and viability results. Brightfield cell counting, fluorescence cell counting, yeast cell counting, and GFP transfection assays are all in the LUNA-FL™ wheelhouse. This versatile little counter sits comfortably in a cell culture hood or on your benchtop to fit seamlessly into your workflow.
What the Luna-FL™ does best
TWO COUNTERS IN ONE
The LUNA-FL™ can count in both brightfield and fluorescence mode, making it Logos' most versatile counter to date.
COMPATIBLE WITH MOST CELLS
Advanced imaging software and GFP and RFP fluorescence optics sensitively detect fluorescence stained cells.
GFP TRANSFECTION ANALYSIS
High resolution images are captured and analyzed to detect GFP-positive and -negative cells.
MAXIMUM 30 SECONDS
Fluorescence: 30 s = 3 images captured & analyzed;
Brightfield: 10 s = 1 image captured & analyzed
THE LOWEST COST. THE GREATEST CONVENIENCE.
Perfect for labs on a budget, our LUNA™ family of automated cell counters work with the LUNA™ Reusable Slide. The LUNA™ Reusable Slide delivers the affordability of manual cell counting without the associated time, subjectivity, and user-to-user variability. Our disposable slides offer the ultimate counting experience. With no mess or cleanup, these precision slides are convenient and maintain the highest standard of cell counting accuracy.
Supplier
Logos Biosystems Inc.
Multimedia
Experimental condition
HL-60 (50% viability)
HL-60 cells were artificially killed by heating and mixed with healty cells (50:50). Mixed cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.
Experimental condition
HL-60 (high density)
A high density culture of HL-60 cells was counted with the LUNA-FL to verifiy the performance of the instrument. Cells were stained with acridine orange-propidium idodide mixture and then counted. Green and red circle represents viable and dead nucleated cells automatically classified by LUNA-FL.
Human peripheral blood
Human whole blood was collected from a finger tip and diluted in PBS at 1:100. After staining with the AO/PI dyes, white blood cells (WBCs) were counted with the LUNA-FL fluorescence cell counter. Bright field and its corresponding fluorescence image are shown in the left and middle panel, respectively. Area enclosed by the white rectangle is shown in the right panel at high magnification. Note that cells containing nucleic acids (ie., WBCs) were counted and tagged with the green circle. A huge number of red blood cells (RBCs) and platelets seen in the bright field image were not counted by LUNA-FL. No dead WBC was observed in this field of view.
Experimental condition
Jurkat
Jurkat T cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.
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