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Mission Bio Tapestri®

Untangle the complexity of cancer with true multi-omics.

The Tapestri Platform is the only system capable of simultaneously providing both genotype and phenotype data from the same cell, across thousands of single cells. With DNA as a foundation, our proprietary two-step microfluidic workflow enables the simultaneous detection of analytes, including SNVs, CNVs, and proteins. Meet the complexity of cancer with true multi-omics analysis.

Gain the true single-cell picture to enable precise understanding

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Precise and Powerful

Tapestri sits on your bench top to scale single-cell DNA analysis as never before

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  • High throughput: Up to 10,000 cells
  • Multi-omic SNV, indel, CNV and protein data from the same single cell
  • Detect rare subclones down to 0.1%
  • Identify mutation co-occurrence and zygosity
  • Small footprint of one square foot

Clonal Resolution with Single-Cell Precision

Complex disease evolves, so understanding genetic variability — including mutation co-occurrence at the single-cell level — is vitally important for clinical researchers to break the cycle of treatment response, resistance and relapse.

Novel Droplet Microfluidics Power Our Platform

The Tapestri Instrument partitions thousands of cells into individual droplets, which act like mini reaction tubes enabling thousands of individual reactions simultaneously. Cell lysis and protease digestion then follow off-instrument enabling access to DNA and protein for downstream amplification.

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Evolving subclonal mutations can resist and escape treatment

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The industry’s first scalable, customizable, single-cell DNA analysis platform.

The Mission Bio Tapestri Platform was developed to help advance precision medicine by enabling the accelerated and accessible detection of genomic variability within and across cell populations. Leveraging proprietary droplet microfluidics, the platform unlocks access to DNA at the single-cell level with a novel two-step protease workflow. This methodology provides flexibility for additional applications and customization capability.

Break Out of the Limitations of Bulk.

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The average read-out from conventional bulk sequencing misses the underlying genetic diversity across cell populations. To improve patient stratification, therapy selection, and disease monitoring we need insights into mutation co-occurrence within every single cell.

Single-Cell DNA Applications

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Hematology

Clonal evolution is foundational to disease progression in hematologic malignancies which can impact therapy response, resistance, relapse and residual disease. Tapestri Single-Cell DNA Panels have fixed content to address the most important hotspot SNVs and indels implicated in AML, MDS, MPN, CML, CMML, and JMML. CNV, LOH and protein analysis can also be integrated into your single-cell data for a true multi-omic readout.

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Solid Tumor

Cellular heterogeneity in solid tumor cancers impacts clonal evolution and patient outcomes. Single-cell DNA and protein solid tumor profiling enables high resolution of the genomic diversity in a variety of tumor types.

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Genome Editing

Advances in genome editing have transformed our ability to deliver precision therapies by engineering specific sequence edits in almost any cell in any genome. While powerful, these genome edits are not always accurate or efficient, and precision tools are needed to provide quality control in ensuring the appropriate edits have been incorporated. Tapestri Single-Cell Custom DNA Panels provides single-cell resolution to assess targeting efficiency of genome editing constructs.

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Indels

Because bulk misses the diversity that matters.

The average read-out from conventional bulk sequencing misses the rare events and underlying genetic diversity within and across cell populations. The Tapestri Platform is the first and only single-cell platform to simultaneously detect Copy Number Variants (CNVs) and Single Nucelotide Variants (SNVs) from the same cells. To resolve heterogeneity and improve patient stratification, therapy selection, and disease monitoring we need to detect rare clonal populations and identify co-occurrence and zygosity within every single cell.

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CNVs

Because the change in our blueprint is where cancer begins.

The only single-cell targeted DNA solution that provides both copy number variants (CNVs) and single nucleotide variants (SNVs) from the same cell. Characterize genomic heterogeneity using a single platform, with comprehensive single-cell DNA analysis to co-detect both SNVs and CNVs. 

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Phenotpye

Because the complexity of cancer needs both.

Introducing the first and only single-cell multi-omic platform capable of detecting SNVs, CNVs, and protein changes simultaneously from the same cell. Gain a true multi-omic picture from genotype to phenotype for your critical cancer research.

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Tapestri Single-Cell DNA Panels

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The accurate and efficient single-cell detection of SNVs, indels, CNVs and LOH across targeted gene panels is now possible with Tapestri Single-Cell DNA Panels.

Hematology

  • Tapestri Single-Cell DNA CLL Panel
  • Tapestri Single-Cell DNA Acute Myeloid Leukemia Panel
  • Tapestri Single-Cell DNA Myeloid Panel

Solid Tumor Profiling

  • Tapestri Single-Cell Tumor Hotspot Panel
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Tapestri Designer for Custom Single-Cell DNA Panels

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The only customizable single-cell targeted DNA solution that allows scientists to reveal the true clonal heterogeneity in research samples.

  • Maximum flexibility to study specific SNVs, indels, CNVs and LOH relevant to your research.

Focus your time and budget with targeted custom panels relevant to your research

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Videos

News & Testimonials

News

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Mission Bio Tapestri® Update

Mission Bio's New Single-Cell Research Platform Marries Sequencing, Cell Surface Protein Analysis

With the launch this week of its new Tapestri platform, single-cell omics firm Mission Bio is looking to make further inroads into the clinical trial and drug development businesses.

The new system adds protein measurements to the company's traditional focus on single-cell DNA sequencing, allowing researchers to make DNA and protein measurements in a single experiment and in single cells.

The system uses BioLegend's TotalSeq antibodies, which are conjugated to oligonucleotides, allowing them to be read out via single-cell sequencing. The current version of the Tapestri platform is intended for analysis of cell surface proteins, which enables researchers to identify cell type and subtype, generating data analogous to that provided by flow cytometry.

Read the full Press Release

- Catherine Smith, MD, UCSF (Helen Diller Family Comprehensive Cancer Center)

"No technology prior to Tapestri has been able to give us the resolution to decipher co-occurring mutations in the same clones."

"These insights are especially important in our study of resistance mechanisms and combinatorial therapies in key clinical trials."

Koichi Takahashi, MD – The University of Texas (MD Anderson Cancer Center)

"This new capability of single-cell analysis is critical to better understanding precision therapy selection at diagnosis as well as disease monitoring and subsequent treatment strategy.”

Resources

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Single-cell Sequencing 101 E-Book
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Mission Bio

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Measure mitochondrial respiration and glycolysis as well as ATP production rate of live cells.

Measure mitochondrial respiration, glycolysis, and ATP production in live cells in a 96-well format

Overview of Consumables for XF Analyzers

Simple, Automated, Charge-based DNA and RNA Extraction

LUNA-FX7™ Automated Fluorescence Cell Counter with unmatched cell counting accuracy

LUNA-III™ Automated Brightfield Cell Counter, designed to enhance your productivity and precision.

High multiplexing, high resolution in situ platform to combine single-cell and spatial genomics.

The best super-resolution microscopy experience, on your desktop.

NovoCyte Opteon Spectral Flow Cytometer Systems 3-5 Lasers and 73 detectors

The NovoCyte Penteon™ is a 5 laser benchtop flow cytometer with up to 30 colors

Pixel – Visualize live cell function at scale

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Benchtop Microfluidic Cell Sorting with Two Lasers and up to Nine Colors

An incubator designed specifically for the cultivation of primary human cells

Seahorse XFe24 Analyzers measure OCR and ECAR of live cells in a 24-well plate format.

Automated high content image acquisition and analysis for drug discovery and cell biology

Multicolor fluorescence imaging to data analysis in one device

IsoLight – Automated Cellular Proteomics Hub

IsoLight and IsoSpark – Single-Cell Functional Proteomics Hub

The LUNA-FL™ can count in both brightfield and fluorescence mode

The LUNA-II™ automated cell counter gives you the ultimate brightfield cell counting experience.

The MARS® System is the “one-stop” modular solution for complete cell isolation from complex samples

The NovoCyte Quanteon™ is a 4 laser benchtop flow cytometer with up to 25 colors

The QUANTOM Tx Microbial Cell Counter automatically identifies and counts individual bacterial cells

Single Cells or Nuclei from Solid Tissues in Minutes

Tapestri® Platform: Untangle the complexity of cancer with true multi-omics.

Sterile, easy-to-use solutions for high cell integrity of single cell dispensing

Tissue Clearing System for electrophoretic tissue clearing

Optimized Microplate Solutions for Research & Drug Discovery

DeepLabel™ Antibody Staining Kit for Cleared Tissues

IsoPlexis Solutions and Kits

Autofocusing cell counting and declustering algorithm that counts individual yeast cells

Automated Fluorescence Cell Counter for Stem Cells & SVF

The NovoCyte Advanteon provides the flexibility of 1, 2, or 3 laser options.

Accurate & efficient single-cell detection of SNVs, indels, CNVs and LOH across targeted gene panels

Measure functional metabolism in 3 dimensions from in vitro spheroids

NovoCyte Consumables and Accessories for your Flow Cytometry needs.

Kits and reagents for real-time fluorescence plate reader-based live cell metabolism assays

Sterile, easy-to-use solutions for high cell integrity of single cell dispensing

The best value benchtop flow cytometer with high flexibility, enhanced sensitivity and resolution.

Optimized solution for assessing mitochondrial function.

Provides real-time measurements of glycolysis

Measures human (Hu) T cell activation response within several minutes of stimulation

Formulated media based on common culture media DMEM or RPMI, and optimized for use in XF assays.

Analytical method for measuring basal state mitochondrial fuel oxidation in live cells

Identification of drug-induced mitochondrial toxicity

Provides validated reagents to measure long chain fatty acid oxidation (FAO)

For in vitro studies of long chain fatty acid oxidation by intact cells.

Permeabilizes intact cells in culture, selectively targeting the cellular plasma membrane.

Seahorse XF Real-Time ATP Rate Assay

A suite of optimized solutions for rapidly measuring cellular substrate oxidation

Robust & accurate measurements of both glycolytic and mitochondrial activities in T cell populations

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