• SMALL: At 2 cubic feet and 54 pounds, researchers will not need to worry about the system taking up precious lab space.
• GOES ANYWHERE: The WOLF G2 is a simple, benchtop system that can easily be moved from lab-to-lab, and fits into biosafety cabinets.
• INTUITIVE SOFTWARE: Unlike traditional cell sorter software, easy to use and requires minimal training.

At < 2 psi, the WOLF G2 are gentler than any conventional cell sorters, enabling healthier cells post-sort, especially for engineered lines, primary cells, and stem cells.

All laser configurations afford < 250 MESF sensitivity, along with forward and back scatter, providing as low as 1 μm resolution.

At 2 cubic feet, NanoCellect’s benchmark for access and performance allows every lab for the flexibility to do analysis and sorting into tubes or 96- and 384-well plates.

Intuitive software, fixed optics, no fluidics cart and less than one minute clean-up time.

Disposable, aerosol-free microfluidic cartridge allows for sterile sorting that protects the sample from the environment and scientist from the sample.

With two lasers and up to nine fluorescent channels, the WOLF G2 aligns with a broad set of research applications and experiments. Three different laser configurations allows options specific to your needs.

NanoCellect utilizes unique and proprietary microfluidic sorting cartridges for bulk sorting and single-cell dispensing. The cartridges employ a piezoelectric actuator and operates at less than 2 psi allows for gentle sorting that preserves viability and RNA integrity. Cartridges have a 100% disposable fluidic pathway, making it ideal for labs with many samples where cross-contamination or biohazard cleanup is an issue. The microfluidic cartridges are sterile and produce no aerosols, making it some of the safest sorting on the market. The cartridges also allow for maximum flexibility by allowing users to sort using their own buffers and media and only consume about 50 mL of sheath fluid in 1 day.

• WOLFViewer software walks users though a simple workflow by offering step-by-step wizards to make the sorting process as straightforward as possible. As a result, the software uses an intuitive workflow allowing users to quickly complete setup.
• After setup, WOLFViewer makes analyzing and creating gates simple—while still providing full-featured flow cytometry tools.
• Key tools like automatic compensation and index sorting, are streamlined and included.
• When sorting is complete, shutdown and cleanup is literally less than a minute, allowing users to rapidly conclude their work.
• Researchers have the option of 2-way sorts for bulk sorting and 1-way sorts for single-cell dispensing through the WOLFViewer software.

Sample preparation is a significant yet underappreciated step that allows for high quality sequencing and analysis, critical to achieving breakthroughs in medicine, agriculture, and other industries. NanoCellect’s microfluidic cell sorter, the WOLF, allow the cell’s integrity to be maintained for numerous sequencing and multi-omics techniques. The WOLF’s ability to flexibly fit into any genomic researcher’s lab, make it a key step to any cell sequencing analysis workflow.

• 10x Genomics RNA-seq library prep
• Single-Cell sorting on PCR plate
• Whole-genome sequencing methods

The antibody drug discovery process differs from the traditional drug discovery of small molecules as it requires the development of hybridomas and the engineering of antibodies to recognize a specific target protein. The production of recombinant antibodies in cancer treatment as well as the development of neutralizing antibodies for infectious diseases, has allowed antibody discovery platforms to interrogate the immune repertoire and develop novel antibody libraries using flow cytometry analysis and cell sorting. The WOLF cell sorter provides a simple, gently cell sorting solution for many workflows such as:

• Antibody Discovery from Single Bovine B-Cells
• Isolate Antibody-Secreting CHO Cells
• Recombinant protein production in CHO Cells

Generating cell lines has often been inefficient and affected by inaccurate limited dilution methods or the shear stress caused by conventional flow cytometer and traditional high-pressure cell sorters. NanoCellect Biomedical provides cell sorting solutions that allow to sort cells and establish stable cell lines using gentle low-pressure microfluidics. The WOLF’s ability to protect cell viability after sorting makes it an integral part of any cell line development workflow such as:

• Recombinant protein production in CHO Cells
• Sorting stem cell cultures
• Antibody discovery

Isolating edited-pluripotent stem cells with traditional sorting techniques can subject the cells to rough conditions in which gene expression, genomic stability and overall cell-health can become compromised. Microfluidic cell sorting with the WOLF, along with single cell deposition using the N1 single cell dispenser, eliminates the challenges that pluripotent stem cells face by sorting at gentle pressures without decompression shock and shear stress, and allow for efficient single cell deposition for high monoclonal outgrowth. Learn more about how cell sorting can improve workflows such as:

• CRISPR and disease modelling
• Protection of Karyotype
• Generation of Genome-Edited iPSCs

A module for the WOLF Cell Sorter to sort and dispense into 96-well or 384-well plates.

The N1 Single Cell Dispenser is seamlessly integrated into the intuitive software. Select single-cell from the dropdown menu, map out your plate, and hit go. The WOLF and dispenser work together to deliver plated cells in a compact and affordable format. After sorting, data from each cell is indexed well-by-well, allowing for confirmation of the plated cell.

• Quickly and effortlessly plate your cells into 96 or 384 well plates.
• Output flexibility allows users to dispense 1 or “n” cells per well.
• Excellent for single-cell cloning or single-cell RNA-seq.